PPM1F antibody (AA 17-454) (Biotin)

Catalog No. ABIN8027976

The Rabbit Polyclonal anti-PPM1F antibody is suitable to detect PPM1F in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for PPM1F antibody (AA 17-454) (Biotin) (ABIN8027976)

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1F antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PPM1F Antibody (Biotin)

Binding Specificity: AA 17-454

Purpose: Anti-PPM1F Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPM1F recombinant protein (Position: E17-S454).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPM1F

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Alternative Name: PPM1F

Background:

Background: Protein phosphatase 1F is an enzyme that in humans is encoded by the PPM1F gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Gene Full Name: protein phosphatase, Mg2+/Mn2+ dependent 1F

Gene ID: 9647

UniProt: P49593

Pathways: Positive Regulation of Endopeptidase Activity