PPM1F antibody (AA 17-454)

Catalog No. ABIN8027986

The Rabbit Polyclonal anti-PPM1F antibody (ABIN8027986) specifically detects PPM1F in WB, ELISA, IHC, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for PPM1F antibody (AA 17-454) (ABIN8027986)

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-PPM1F Antibody

Binding Specificity: AA 17-454

Purpose: Anti-PPM1F Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PPM1F Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPM1F recombinant protein (Position: E17-S454).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPM1F

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Alternative Name: PPM1F

Background:

Background: Protein phosphatase 1F is an enzyme that in humans is encoded by the PPM1F gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Gene Full Name: protein phosphatase, Mg2+/Mn2+ dependent 1F

Molecular Weight: 50 kDa

Gene ID: 9647

UniProt: P49593

Pathways: Positive Regulation of Endopeptidase Activity