RFXANK antibody (AA 29-260)

Catalog No. ABIN8031287

This Rabbit Polyclonal antibody specifically detects RFXANK in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RFXANK antibody (AA 29-260) (ABIN8031287)

Target: RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFXANK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-RFXANK Antibody

Binding Specificity: AA 29-260

Purpose: Anti-RFXANK Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human RFXANK shares 85.1% amino acid (aa) sequence identity with mouse RFXANK.

Characteristics: Anti-RFXANK Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RFXANK recombinant protein (Position: E29-E260). Human RFXANK shares 85.1% amino acid (aa) sequence identity with mouse RFXANK.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RFXANK

Target: RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))

Alternative Name: RFXANK

Background:

Background: DNA-binding protein RFXANK is a protein that in humans is encoded by the RFXANK gene. Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Gene Full Name: regulatory factor X associated ankyrin containing protein

Molecular Weight: 34 kDa

Gene ID: 8625

UniProt: O14593