E2F1 antibody (pSer364) (Biotin)

Catalog No. ABIN753480

The Biotin-conjugated Rabbit Polyclonal anti-E2F1 antibody (ABIN753480) specifically detects E2F1 in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for E2F1 antibody (pSer364) (Biotin) (ABIN753480)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-E2F1 Antibody (Biotin)

Binding Specificity: pSer364

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Ser364

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Synonyms: RBP3, E2F-1, RBAP1, RBBP3, Transcription factor E2F1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1

Background: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy