RECK antibody (AA 179-806)

Catalog No. ABIN8032050

The Rabbit Polyclonal anti-RECK antibody is suitable to detect RECK in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for RECK antibody (AA 179-806) (ABIN8032050)

Target: RECK (Reversion-Inducing-Cysteine-Rich Protein with Kazal Motifs (RECK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RECK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-RECK Antibody

Binding Specificity: AA 179-806

Purpose: Anti-RECK Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human RECK shares 94.3% amino acid (aa) sequence identity with mouse RECK.

Characteristics: Anti-RECK Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RECK recombinant protein (Position: Q179-K806). Human RECK shares 94.3% amino acid (aa) sequence identity with mouse RECK.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RECK

Target: RECK (Reversion-Inducing-Cysteine-Rich Protein with Kazal Motifs (RECK))

Alternative Name: RECK

Background:

Background: Reversion-inducing-cysteine-rich protein with kazal motifs, also known as RECK, is a human gene, thought to be a metastasis suppressor. The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: reversion inducing cysteine rich protein with kazal motifs

Molecular Weight: 100 kDa

Gene ID: 8434

UniProt: O95980