ARHGEF7 antibody (AA 25-637)

Catalog No. ABIN8032193

This Rabbit Polyclonal antibody specifically detects ARHGEF7 in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ARHGEF7 antibody (AA 25-637) (ABIN8032193)

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-ARHGEF7 Antibody

Binding Specificity: AA 25-637

Purpose: Anti-ARHGEF7 Antibody

Predicted Reactivity: Human ARHGEF7 shares 90.4% amino acid (aa) sequence identity with mouse ARHGEF7.

Characteristics: Anti-ARHGEF7 Antibody. Tested in WB, IHC, ICC/IF, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ARHGEF7 recombinant protein (Position: S25-N637). Human ARHGEF7 shares 90.4% amino acid (aa) sequence identity with mouse ARHGEF7.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ARHGEF7

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Alternative Name: ARHGEF7

Background:

Background: Rho guanine nucleotide exchange factor 7 is a protein that in humans is encoded by the ARHGEF7 gene. This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene.

Gene Full Name: Rho guanine nucleotide exchange factor 7

Molecular Weight: 80 kDa

Gene ID: 8874

UniProt: Q14155

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation