RNH1 antibody (N-Term)

Catalog No. ABIN8032414

The Mouse Monoclonal anti-RNH1 antibody (Clone 4F3) (ABIN8032414) specifically detects RNH1 in WB, IHC, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for RNH1 antibody (N-Term) (ABIN8032414)

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: 4F3

Product Details anti-RNH1 Antibody

Binding Specificity: N-Term

Purpose: Anti-Ribonuclease Inhibitor/RNH1 Antibody (monoclonal, 4F3)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by five amino acids,and from the related rat sequence by four amino acids.

Characteristics: Anti-Ribonuclease Inhibitor/RNH1 Antibody.(monoclonal, 4F3). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RNH1, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RNH1

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Alternative Name: RNH1

Background:

Background: Ribonuclease inhibitor is an enzyme that in humans is encoded by the RNH1 gene. Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular Rnases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover, the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.

Gene Full Name: ribonuclease/angiogenin inhibitor 1

Molecular Weight: 45 kDa

Gene ID: 6050

UniProt: P13489