RNF111 antibody (AA 134-994)

Catalog No. ABIN8033173

The Rabbit Polyclonal anti-RNF111 antibody (ABIN8033173) specifically detects RNF111 in WB, ELISA, FACS, IHC, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RNF111 antibody (AA 134-994) (ABIN8033173)

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF111 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-RNF111 Antibody

Binding Specificity: AA 134-994

Purpose: Anti-RNF111 Antibody

Characteristics: Anti-RNF111 Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF111 recombinant protein (Position: F134-S994).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF111

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Alternative Name: RNF111

Background:

Background: E3 ubiquitin-protein ligase Arkadia is an enzyme that in humans is encoded by the RNF111 gene. The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Gene Full Name: ring finger protein 111

Molecular Weight: 109 kDa

Gene ID: 54778

UniProt: Q6ZNA4