RNF43 antibody (AA 24-197) (HRP)

Catalog No. ABIN8033345

This Rabbit Polyclonal antibody specifically detects RNF43 in ELISA, WB and IHC. It exhibits reactivity toward Human.

Quick Overview for RNF43 antibody (AA 24-197) (HRP) (ABIN8033345)

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF43 antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RNF43 Antibody (HRP)

Binding Specificity: AA 24-197

Purpose: Anti-RNF43 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF43

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Alternative Name: RNF43

Background:

Background: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: ring finger protein 43

Gene ID: 54894

UniProt: Q68DV7

Pathways: WNT Signaling