RNF43 antibody (AA 24-197)

Catalog No. ABIN8033349

The Rabbit Polyclonal anti-RNF43 antibody is suitable to detect RNF43 in samples from Human. It has been validated for ELISA and WB.

Quick Overview for RNF43 antibody (AA 24-197) (ABIN8033349)

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF43 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Carrier-free

Product Details anti-RNF43 Antibody

Binding Specificity: AA 24-197

Purpose: Anti-RNF43 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RNF43 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).

Isotype: IgG

Application Details

Application Notes: Western blot,0.1-0.5 μg/mL ELISA,0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RNF43

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Alternative Name: RNF43

Background:

Background: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: ring finger protein 43

Molecular Weight: 86 kDa

Gene ID: 54894

UniProt: Q68DV7

Pathways: WNT Signaling