NeuN antibody (AA 28-304)

Catalog No. ABIN8033785

The Rabbit Polyclonal anti-NeuN antibody (ABIN8033785) specifically detects NeuN in WB, IHC, ELISA and IF. The antibody is reactive with Mouse and Rat samples.

Quick Overview for NeuN antibody (AA 28-304) (ABIN8033785)

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NeuN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-NeuN Antibody

Binding Specificity: AA 28-304

Purpose: Anti-NeuN/Rbfox3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NeuN/Rbfox3 Antibody. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse NeuN/Rbfox3 recombinant protein (Position: H28-D304).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunofluorescence, 5 μg/mL, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Alternative Name: Rbfox3

Background:

Background: RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) is a protein that in humans is encoded by the RBFOX3 gene. This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Gene Full Name: RNA binding protein, fox-1 homolog (C. elegans) 3

Molecular Weight: 46-55 kDa

Gene ID: 52897

UniProt: Q8BIF2