POLR2A/RPB1 antibody (AA 10-321) (FITC)

Catalog No. ABIN8033835

The Rabbit Polyclonal anti-POLR2A/RPB1 antibody is suitable to detect POLR2A/RPB1 in samples from Human, Mouse, Rat and Monkey. It has been validated for FACS.

Quick Overview for POLR2A/RPB1 antibody (AA 10-321) (FITC) (ABIN8033835)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-POLR2A/RPB1 Antibody (FITC)

Binding Specificity: AA 10-321

Purpose: Anti-RNA polymerase II/POLR2A Antibody FITC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNA polymerase II/POLR2A recombinant protein (Position: D10-E321).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A

Background:

Background: DNA-ed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. It is mapped to 17p13.1. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Gene Full Name: RNA polymerase II subunit A

Gene ID: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways