ASH2L antibody (AA 142-628) (PE)

Catalog No. ABIN8036785

The PE-conjugated Rabbit Polyclonal anti-ASH2L antibody (ABIN8036785) specifically detects ASH2L in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ASH2L antibody (AA 142-628) (PE) (ABIN8036785)

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASH2L antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-ASH2L Antibody (PE)

Binding Specificity: AA 142-628

Purpose: Anti-ASH2L Antibody PE Conjugated

Predicted Reactivity: Human ASH2L shares 99.2% amino acid (aa) sequence identity with mouse ASH2L.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ASH2L recombinant protein (Position: N142-P628). Human ASH2L shares 99.2% amino acid (aa) sequence identity with mouse ASH2L.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for ASH2L

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Alternative Name: ASH2L

Background:

Background: Set1/Ash2 histone methyltransferase complex subunit ASH2 is an enzyme that in humans is encoded by the ASH2L gene. The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator. While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4. These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity. MLL1 and MLL2 complexes contain the additional protein subunit, menin. Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4. MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis.

Gene Full Name: ASH2 like, histone lysine methyltransferase complex subunit

Gene ID: 9070

UniProt: Q9UBL3