Shugoshin antibody (N-Term)

Catalog No. ABIN8037109

The Rabbit Polyclonal anti-Shugoshin antibody (ABIN8037109) specifically detects Shugoshin in WB, IHC and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for Shugoshin antibody (N-Term) (ABIN8037109)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Shugoshin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Shugoshin Antibody

Binding Specificity: N-Term

Purpose: Anti-SGOL1/SGO1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SGOL1/SGO1 Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SGOL1/SGO1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Alternative Name: SGO1

Background:

Background: The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

Gene Full Name: shugoshin 1

Molecular Weight: 75 kDa

Gene ID: 151648

UniProt: Q5FBB7

Pathways: Maintenance of Protein Location