CD43 antibody (AA 272-400) (APC)

Catalog No. ABIN8037216

The Rabbit Polyclonal anti-CD43 antibody is suitable to detect CD43 in samples from Human, Mouse and Rat. It has been validated for FACS, WB, IHC, IF, IHC (fro) and ICC.

Quick Overview for CD43 antibody (AA 272-400) (APC) (ABIN8037216)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD43 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-CD43 Antibody (APC)

Binding Specificity: AA 272-400

Purpose: Anti-CD43/SPN Antibody APC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human CD43 shares 72%,73% amino acid (aa) sequences identity with mouse,rat CD43,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry, 0.5-1 μg/mL, Human, - Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: SPN

Background:

Background: CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

Gene Full Name: sialophorin

Sequence Similarities: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity