CD43 antibody (AA 272-400) (PE)

Catalog No. ABIN8037228

The Mouse Monoclonal anti-CD43 antibody is suitable to detect CD43 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for CD43 antibody (AA 272-400) (PE) (ABIN8037228)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 4I3

Product Details anti-CD43 Antibody (PE)

Binding Specificity: AA 272-400

Purpose: Anti-CD43/SPN Antibody (monoclonal, 4I3) PE Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human CD43 shares 72%,73% amino acid (aa) sequence identity with mouse,rat CD43,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequence identity with mouse and rat CD43, respectively.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: SPN

Background:

Background: CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

Gene Full Name: sialophorin

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity