SIX3 antibody (N-Term) (Fluoro488)

Catalog No. ABIN8037708

The Rabbit Polyclonal anti-SIX3 antibody is suitable to detect SIX3 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for SIX3 antibody (N-Term) (Fluoro488) (ABIN8037708)

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIX3 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-SIX3 Antibody (Fluoro488)

Binding Specificity: N-Term

Purpose: Anti-Six3 Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by two amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Six3, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Alternative Name: SIX3

Background:

Background: Homeobox protein SIX3 is a protein that in humans is encoded by the SIX3 gene. This gene encodes a member of the sine oculishomeobox transcription factor family. The encoded protein plays a role in eye development. Mutations in SIX3 are the cause of a severe brain malformation, called holoprosencephaly type 2 (HPE2). In HPE2, the brain fails to separate into two hemispheres during early embryonic development, leading to eye and brain malformations, which result in serious facial abnormalities. A mutant zebrafish knockout model has been developed, in which the anterior part of the head was missing due to the atypical increase of Wnt1 activity. When injected with SIX3, these zebrafish embryos were able to successfully develop a normal forebrain. When SIX3 was turned off in mice, resulting in a lack of retina formation due to excessive expression of Wnt8b in the region where the forebrain normally develops. Both of these studies demonstrate the importance of SIX3 activity in brain and eye development.

Gene Full Name: SIX homeobox 3

Gene ID: 6496

UniProt: O95343

Pathways: Protein targeting to Nucleus