SMAD7 antibody (AA 353-388)

Catalog No. ABIN8038212

The Rabbit Polyclonal anti-SMAD7 antibody is suitable to detect SMAD7 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for SMAD7 antibody (AA 353-388) (ABIN8038212)

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-SMAD7 Antibody

Binding Specificity: AA 353-388

Purpose: Anti-MADH7/SMAD7 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MADH7/SMAD7 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MADH7/SMAD7 recombinant protein (Position: R353-Q388).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SMAD7

Target: SMAD7 (SMAD, Mothers Against DPP Homolog 7 (SMAD7))

Alternative Name: SMAD7

Background:

Background: Mothers against decapentaplegic homolog 7 or SMAD7 is a protein that in humans is encoded by the SMAD7 gene. The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: SMAD family member 7

Molecular Weight: 50 kDa

Gene ID: 4092

UniProt: O15105

Pathways: Interferon-gamma Pathway, Cell-Cell Junction Organization