HSC70 Interacting Protein HIP antibody (N-Term) (Biotin)

Catalog No. ABIN8043679

The Biotin-conjugated Rabbit Polyclonal anti-HSC70 Interacting Protein HIP antibody (ABIN8043679) specifically detects HSC70 Interacting Protein HIP in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for HSC70 Interacting Protein HIP antibody (N-Term) (Biotin) (ABIN8043679)

Target: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSC70 Interacting Protein HIP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-HSC70 Interacting Protein HIP Antibody (Biotin)

Binding Specificity: N-Term

Purpose: Anti-HSC70 Interacting Protein HIP/ST13 Antibody Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by two amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ST13, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HSC70 Interacting Protein HIP

Target: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Alternative Name: ST13

Background:

Background: ST13 (Suppression of Tumorigenicity 13), also known as P48 or HIP, is a protein that in humans is encoded by the ST13 gene. ST13 is an abundant, highly conserved protein that binds the major cytosolic chaperones heat-shock protein 70-kD (HSP70) and HSP90 during an intermediate stage of steroid receptor assembly, but is absent from the mature receptor complex. Zhang et al. (1998) mapped the ST13 gene to chromosome 22q13 by fluorescence in situ hybridization. They noted that colorectal, breast, and ovarian carcinomas frequently show loss of heterozygosity at this site. Using a yeast 2-hybrid assay, Hohfeld et al. (1995) showed that rat Hip bound Hsc70 (HSPA8). One Hip oligomer bound the ATPase domains of at least 2 Hsc70 Molecules, and binding was dependent on activation of the Hsc70 ATPase by Hsp40 (DNAJB1). Hip stabilized the ADP-bound form of Hsc70, which had a high affinity for a test protein substrate. Hohfeld et al. (1995) concluded that HIP contributes to interactions of HSC70 with target proteins through its own chaperone activity.

Gene Full Name: ST13 Hsp70 interacting protein

Sequence Similarities: Belongs to the FAM10 family.

Gene ID: 6767

UniProt: P50502