SYNPO antibody (C-Term)

Catalog No. ABIN8044416

The Rabbit Polyclonal anti-SYNPO antibody is suitable to detect SYNPO in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS, ICC and IF.

Quick Overview for SYNPO antibody (C-Term) (ABIN8044416)

Target: SYNPO (Synaptopodin (SYNPO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYNPO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-SYNPO Antibody

Binding Specificity: C-Term

Purpose: Anti-Synaptopodin/SYNPO Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Synaptopodin/SYNPO Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptopodin/SYNPO, which shares 100% and 94.3% amino acid (aa) sequence identity with mouse and rat Synaptopodin/SYNPO, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SYNPO

Target: SYNPO (Synaptopodin (SYNPO))

Alternative Name: SYNPO

Background:

Background: The spine apparatus (SA) is a specialized form of endoplasmic reticulum (ER) that is found in a subpopulation of dendritic spines in central neurons. The SA consists of a series of stacked discs that are though to be connected to each other and to the dendritic system of ER-tubules. The actin binding protein synaptopodin (which has originally been described in podocytes of the kidney) is an essential component of the SA. Mice that lack the gene for synaptopodin do not form a spine apparatus. The SA is believed to play a critical role in learning and memory. In summary, an important function of the spine apparatus is the regulation of plasticity at individual synapses, a process known as metaplasticity. The International Radiation Hybrid Mapping Consortium mapped the SYNPO gene to chromosome 5.

Gene Full Name: synaptopodin

Molecular Weight: 99 kDa

Gene ID: 11346

UniProt: Q8N3V7