T-Box 5 antibody (AA 253-518)

Catalog No. ABIN8045067

The Rabbit Polyclonal anti-T-Box 5 antibody is suitable to detect T-Box 5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS and IF.

Quick Overview for T-Box 5 antibody (AA 253-518) (ABIN8045067)

Target: T-Box 5 (TBX5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Box 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-T-Box 5 Antibody

Binding Specificity: AA 253-518

Purpose: Anti-TBX5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human TBX5 shares 94.7%,94.4% amino acid (aa) sequence identity with mouse,rat TBX5,respectively.

Characteristics: Anti-TBX5 Antibody. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TBX5 recombinant protein (Position: K253-S518). Human TBX5 shares 94.7% and 94.4% amino acid (aa) sequence identity with mouse and rat TBX5, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for T-Box 5

Target: T-Box 5 (TBX5)

Alternative Name: TBX5

Background:

Background: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

Gene Full Name: T-box transcription factor 5

Molecular Weight: 65 kDa

Gene ID: 6910

UniProt: Q99593