TBC1D4 antibody (AA 1118-1298)

Catalog No. ABIN8045562

The Rabbit Polyclonal anti-TBC1D4 antibody is suitable to detect TBC1D4 in samples from Human. It has been validated for WB, ELISA, IHC, IF, FACS and ICC.

Quick Overview for TBC1D4 antibody (AA 1118-1298) (ABIN8045562)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-TBC1D4 Antibody

Binding Specificity: AA 1118-1298

Purpose: Anti-AS160/TBC1D4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AS160/TBC1D4 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TBC1D4 recombinant protein (Position: K1118-P1298).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: TBC1D4

Background:

Background: AS160 (Akt substrate of 160 kDa), which was originally known asTBC1 domain family member 4 (TBC1D4),is a RabGTPase-activatingproteinthat in humans is encoded by theTBC1D4gene. This gene is a member of the Tre-2/BUB2/CDC16 domain family. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion.

Gene Full Name: TBC1 domain family member 4

Molecular Weight: 160 kDa

Gene ID: 9882

UniProt: O60343