Thrombopoietin antibody (AA 22-258)

Catalog No. ABIN8046345

The Rabbit Polyclonal anti-Thrombopoietin antibody is suitable to detect Thrombopoietin in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Thrombopoietin antibody (AA 22-258) (ABIN8046345)

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Carrier-free

Product Details anti-Thrombopoietin Antibody

Binding Specificity: AA 22-258

Purpose: Anti-Thrombopoietin/THPO Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human Thrombopoietin,TPO recombinant protein (Position: S22-R258). Human Thrombopoietin,TPO shares 79.3%,80.6% amino acid (aa) sequence identity with mouse,rat Thrombopoietin,TPO,respectively.

Characteristics: Anti-Thrombopoietin/THPO Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Thrombopoietin/TPO recombinant protein (Position: S22-R258). Human Thrombopoietin/TPO shares 79.3% and 80.6% amino acid (aa) sequence identity with mouse and rat Thrombopoietin/TPO, respectively.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -Western blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: THPO

Background:

Background: Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.

Gene Full Name: thrombopoietin

Molecular Weight: 45 kDa

Gene ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity