TJP1 antibody (AA 1178-1527)

Catalog No. ABIN8046741

The Rabbit Polyclonal anti-TJP1 antibody is suitable to detect TJP1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS, IF and ICC.

Quick Overview for TJP1 antibody (AA 1178-1527) (ABIN8046741)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-TJP1 Antibody

Binding Specificity: AA 1178-1527

Purpose: Anti-ZO1 tight junction protein/TJP1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1.

Characteristics: Anti-ZO1 tight junction protein/TJP1 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TJP1 recombinant protein (Position: H1178-F1527). Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: TJP1

Background:

Background: Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. It is mapped to 15q13.1. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex.

Gene Full Name: tight junction protein 1

Sequence Similarities: Belongs to the MAGUK family.

Molecular Weight: 220 kDa

Gene ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization