RPH3AL antibody (AA 25-130) (Cy5)

Catalog No. ABIN755057

The Rabbit Polyclonal anti-RPH3AL antibody is suitable to detect RPH3AL in samples from Mouse. It has been validated for WB, IF (cc) and IF (p).

Quick Overview for RPH3AL antibody (AA 25-130) (Cy5) (ABIN755057)

Target: RPH3AL (Rabphilin 3A-Like (Without C2 Domains) (RPH3AL))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPH3AL antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-RPH3AL Antibody (Cy5)

Binding Specificity: AA 25-130

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RPH3AL

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RPH3AL

Target: RPH3AL (Rabphilin 3A-Like (Without C2 Domains) (RPH3AL))

Alternative Name: RPH3AL

Background:

Synonyms: Noc2, Rab effector Noc2, Rabphilin 3A-like without C2 domains, Rabphilin 3A-like, Rabphilin-3A-like protein, RPH3AL, RPH3L_HUMAN, No C2 domains protein, Gm1753.

Background: Rabphilin-3AL (rabphilin-3A-like), also known as RPH3AL or NOC2, is a cytoplasmic Rab GTPase effector. It contains one FYVE-type zinc finger and one Rab-binding (RBD) domain, but unlike its related protein, rabphilin-3A, rabphilin-3AL does not contain any C2 domains. Rabphilin-3AL is expressed in a variety of tissues, with highest levels found in kidney, skeletal muscle, pancreas, liver, ovary, stomach, heart and thyroid. It is believed to play a role regulating calcium-dependent secretory vesicle exocytosis in endocrine and exocrine cells. Via its RBD domain, rabphilin-3AL is capable of binding Rab 27a and, through this interaction, rabphilin-3AL is recruited to dense-core vesicles. With lower affinity, rabphilin-3AL can also bind Rab 3 and Rab 8 with its RBD domain. Through an interaction with Rab 3, rabphilin-3AL can inhibit G-protein signaling in endocrine pancreas and positively regulate insulin secretion. Rabphilin-3AL knockout mice display accumulation of secretory granules and irregular shape in exocrine cells.

Gene ID: 9501

Pathways: Carbohydrate Homeostasis, Regulation of G-Protein Coupled Receptor Protein Signaling