U2AF2 antibody (AA 238-470)

Catalog No. ABIN8051907

The Mouse Monoclonal anti-U2AF2 antibody is suitable to detect U2AF2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for U2AF2 antibody (AA 238-470) (ABIN8051907)

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This U2AF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: 10F4

Product Details anti-U2AF2 Antibody

Binding Specificity: AA 238-470

Purpose: Anti-U2AF65/U2AF2 Antibody (monoclonal, 10F4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-U2AF65/U2AF2.Antibody (monoclonal, 10F4). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human U2AF65/U2AF2 recombinant protein (Position: M238-H470).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for U2AF2

Target: U2AF2 (U2AF59) (U2 Small Nuclear RNA Auxiliary Factor 2 (U2AF59))

Alternative Name: U2AF2

Background:

Background: Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Gene Full Name: U2 small nuclear RNA auxiliary factor 2

Molecular Weight: 65 kDa

Gene ID: 11338

UniProt: P26368