YBX1 antibody (Middle Region)

Catalog No. ABIN8054865

The Rabbit Polyclonal anti-YBX1 antibody is suitable to detect YBX1 in samples from Rat and Mouse. It has been validated for WB and FACS.

Quick Overview for YBX1 antibody (Middle Region) (ABIN8054865)

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YBX1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-YBX1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-YB1/Ybx1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related rat sequences.

Characteristics: Anti-YB1/Ybx1 Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse YB1/Ybx1, identical to the related rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: Ybx1

Background:

Background: Y box binding protein 1 also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1 is a protein that in humans is encoded by the YBX1 gene. Enables chromatin binding activity, mRNA binding activity, and single-stranded DNA binding activity. Involved in epidermis development and negative regulation of cellular senescence. Acts upstream of or within several processes, including cellular response to interleukin-7, negative regulation of striated muscle cell differentiation, and negative regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Part of histone pre-mRNA 3'end processing complex. Is expressed in several structures, including branchial arch, central nervous system, facial prominence, gonad, and retina. Orthologous to human YBX1 (Y-box binding protein 1).

Gene Full Name: Y box protein 1

Molecular Weight: 50 kDa

Gene ID: 22608

UniProt: P62960

Pathways: Regulation of Muscle Cell Differentiation