Zona Pellucida Glycoprotein 3 antibody (Middle Region) (Fluoro647)

Catalog No. ABIN8055370

The Fluoro647-conjugated Rabbit Polyclonal anti-Zona Pellucida Glycoprotein 3 antibody (ABIN8055370) specifically detects Zona Pellucida Glycoprotein 3 in FACS. The antibody is reactive with Human samples.

Quick Overview for Zona Pellucida Glycoprotein 3 antibody (Middle Region) (Fluoro647) (ABIN8055370)

Target: Zona Pellucida Glycoprotein 3 (ZP3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Zona Pellucida Glycoprotein 3 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-Zona Pellucida Glycoprotein 3 Antibody (Fluoro647)

Binding Specificity: Middle Region

Purpose: Anti-ZP3 Antibody Fluoro647 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ZP3, which shares 77.4% amino acid (aa) sequence identity with both mouse and rat ZP3.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Zona Pellucida Glycoprotein 3

Target: Zona Pellucida Glycoprotein 3 (ZP3)

Alternative Name: ZP3

Background:

Background: Zona pellucida sperm-binding protein 3, also known as zona pellucida glycoprotein 3 (Zp-3) or the sperm receptor, is a ZP module-containing protein that in humans is encoded by the ZP3 gene. It is mapped to 7q11.23. he zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus, however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.

Gene Full Name: zona pellucida glycoprotein 3

Gene ID: 7784

UniProt: P21754

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process