C3 antibody

Catalog No. ABIN8065434

The Mouse Monoclonal anti-C3 antibody is suitable to detect C3 in samples from Human. It has been validated for ELISA and IHC.

Quick Overview for C3 antibody (ABIN8065434)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: D17-1

Product Details anti-C3 Antibody

Purpose: Anti-Complement C3a/C3a(desArg)/C3 Antibody

Specificity: Recognizes an epitope that is present on human C3, C3a and C3a (desArg). Does not cross-react with C4a or C5a. GAU 017-01 recognizes different epitopes on the 9 kDa C3a than GAU 013-16. No reaction is seen with a synthetic octapeptide representing the C3a C-terminal.

Predicted Reactivity: Human

Purification: Protein A or Protein G purified

Immunogen: Human C3a

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C without exposure to light

Expiry Date: 12 months

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3a/C3a

Background: C3, AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, acylation-stimulating protein cleavage product, complement component 3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3,Precursor of non-enzymatic components of the classical, alternative, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. {ECO:0000269|PubMed:12878586, ECO:0000269|PubMed:18204047, ECO:0000269|PubMed:28264884, ECO:0000269|PubMed:31507604, ECO:0000269|PubMed:39814882, ECO:0000269|PubMed:39914456, ECO:0000269|PubMed:624565, ECO:0000269|PubMed:6554279}., [Complement C3b]: Non-enzymatic component of C5 convertase (PubMed:28264884, PubMed:31507604, PubMed:3653927, PubMed:3897448). Generated following cleavage by C3 convertase, it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal (PubMed:28264884, PubMed:31507604, PubMed:3653927, PubMed:3897448, PubMed:833545, PubMed:8349625). Complement C3b binds covalently via its reactive thioester, to cell surface carbohydrates or immune aggregates (PubMed:6903192). Together with complement C4b, it then recruits the serine protease complement C2b to form the C5 convertase, which cleaves and activate C5, the next component of the complement pathways (PubMed:12878586, PubMed:18204047, PubMed:2387864). In the alternative complement pathway, recruits the serine protease CFB to form the C5 convertase that cleaves and activates C5 (PubMed:624565, PubMed:6554279). {ECO:0000269|PubMed:12878586, ECO:0000269|PubMed:18204047, ECO:0000269|PubMed:2387864, ECO:0000269|PubMed:28264884, ECO:0000269|PubMed:31507604, ECO:0000269|PubMed:3653927, ECO:0000269|PubMed:3897448, ECO:0000269|PubMed:624565, ECO:0000269|PubMed:6554279, ECO:0000269|PubMed:6903192, ECO:0000269|PubMed:833545, ECO:0000269|PubMed:8349625}., [C3a anaphylatoxin]: Mediator of local inflammatory process released following cleavage by C3 convertase (PubMed:6968751, PubMed:37169960, PubMed:37852260). Acts by binding to its receptor, C3AR1, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C3AR1 (PubMed:8702752, PubMed:37169960, PubMed:37852260). C3a anaphylatoxin stimulates the activation of immune cells such as mast cells and basophilic leukocytes to release inflammation agents, such as cytokines, chemokines and histamine, which promote inflammation development (PubMed:23383423). Also acts as potent chemoattractant for the migration of macrophages and neutrophils to the inflamed tissues, resulting in neutralization of the inflammatory triggers by multiple ways, such as phagocytosis and generation of reactive oxidants (PubMed:23383423, PubMed:342601, PubMed:5778786). {ECO:0000269|PubMed:342601, ECO:0000269|PubMed:37169960, ECO:0000269|PubMed:37852260, ECO:0000269|PubMed:5778786, ECO:0000269|PubMed:6968751, ECO:0000269|PubMed:8702752, ECO:0000303|PubMed:23383423}., [Acylation stimulating protein]: Adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial triglyceride clearance (PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750, PubMed:2909530, PubMed:8376604, PubMed:9059512). Appears to stimulate triglyceride synthesis via activation of the PLC, MAPK and AKT signaling pathways (PubMed:16333141). Acts by binding to its receptor, C5AR2, activating G protein-coupled receptor signaling, promoting the phosphorylation, ARRB2-mediated internalization and endocytosis of C5AR2 (PubMed:11773063, PubMed:12540846, PubMed:19615750). In contrast to C3a anaphylatoxin peptide, does not show pro-inflammatory activity (PubMed:37852260). {ECO:0000269|PubMed:10432298, ECO:0000269|PubMed:11773063, ECO:0000269|PubMed:12540846, ECO:0000269|PubMed:15833747, ECO:0000269|PubMed:16333141, ECO:0000269|PubMed:19615750, ECO:0000269|PubMed:2909530, ECO:0000269|PubMed:37852260, ECO:0000269|PubMed:8376604, ECO:0000269|PubMed:9059512}., [C3-beta-c]: Acts as a chemoattractant for neutrophils in chronic inflammation. {ECO:0000250|UniProtKB:P01026}.,C3,Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).

Molecular Weight: 187 kDA (Native C3), 9 kDa (C3a and C3a(desArg))

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling