HSPG2 antibody

Catalog No. ABIN8065501

The Mouse Monoclonal anti-HSPG2 antibody (Clone A74) (ABIN8065501) specifically detects HSPG2 in WB, IHC, ELISA, IP and AP. The antibody is reactive with Cow samples.

Quick Overview for HSPG2 antibody (ABIN8065501)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Affinity Purification (AP)

Grade: Carrier-free

Clone: A74

Product Details anti-HSPG2 Antibody

Purpose: Anti-Perlecan Antibody

Specificity: CSI 001-74 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Epitope is located in domain V

Predicted Reactivity: Bovine,Human

Purification: Protein A or Protein G purified

Immunogen: Bovine corneal endothelial cells

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C without exposure to light

Expiry Date: 24 months

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: Perlecan

Background: HSPG2, HSPG, PLC, PRCAN, SJA, SJS, SJS1, basement membrane-specific heparan sulfate proteoglycan core protein, endorepellin (domain V region), perlecan proteoglycan,Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development (PubMed:12435733, PubMed:15591058, PubMed:19789387). In muscle, it is essential for localizing acetylcholinesterase (AChE) at the neuromuscular junctions (NMJ), most probably acting as an adapter that links the acetylcholinesterase collagenic tail peptide (COLQ) to alpha-dystroglycan, and is therefore involved in the down-regulation of colinergic synaptic transmission (By similarity). {ECO:0000250|UniProtKB:Q05793, ECO:0000269|PubMed:12435733, ECO:0000269|PubMed:15591058, ECO:0000269|PubMed:19789387}., [Endorepellin]: Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6., [LG3 peptide]: Has anti-angiogenic properties that require binding of calcium ions for full activity.,HSPG2,Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.

Molecular Weight: 400-450 kDa

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism