TUBB antibody

Catalog No. ABIN8071385

This Mouse Monoclonal antibody specifically detects TUBB in WB, IHC, ICC and IP. It exhibits reactivity toward Human, Mouse and Paramecium.

Quick Overview for TUBB antibody (ABIN8071385)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Paramecium

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: AXO45

Product Details anti-TUBB Antibody

Purpose: anti-beta-Tubulin, mAb (AXO45)

Specificity: Detects almost all isotypes of beta-tubulin specifically in cells and tissues of mouse, human and ciliates.

Characteristics: Monoclonal Antibody. Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates. Applications: ICC, IHC, IP and WB. Clone: AXO45. Isotype: Mouse IgG1. The β-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals.

Purity: >95 % (SDS-PAGE)

Immunogen: Axonemal tubulin from the ciliate Paramecium electroeluted from the 55 kDa band of the SDS-PAGE gel.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-Tubulin

Background:

Alternate Names/Synonyms: Tubulin beta Chain, Tubulin beta-5 Chain

Product Description: The beta-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both alpha- and beta-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of beta-tubulin. Although the antibody was raised against the beta-tubulin found in cilia, it is able to recognize beta-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of beta-tubulin found in mammals.

Pathways: Microtubule Dynamics, M Phase