CDC25B antibody (pSer353) (Biotin)

Catalog No. ABIN756984

This anti-CDC25B antibody is a Rabbit Polyclonal antibody detecting CDC25B in WB, ELISA and IHC (p). Suitable for Human and Mouse.

Quick Overview for CDC25B antibody (pSer353) (Biotin) (ABIN756984)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC25B Antibody (Biotin)

Binding Specificity: pSer353

Specificity: This phosphorylation site is homologous to that of Ser351 in Mouse and Ser 349 in Rat.

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: Cdc25B

Background:

Synonyms: M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2

Background: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Gene ID: 994

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy