CDC27 antibody (pThr244) (Cy5)

Catalog No. ABIN757331

The Rabbit Polyclonal anti-CDC27 antibody has been validated for IF (cc) and IF (p). It is suitable to detect CDC27 in samples from Rat.

Quick Overview for CDC27 antibody (pThr244) (Cy5) (ABIN757331)

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC27 antibody is conjugated to Cy5

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CDC27 Antibody (Cy5)

Binding Specificity: pThr244

Cross-Reactivity: Rat

Predicted Reactivity: Human,Dog,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CDC27 around the phosphorylation site of Thr244

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Alternative Name: CDC27

Background:

Synonyms: ANAPC3, Anaphase Promoting Complex 3, Anaphase promoting complex protein 3, Anaphase Promoting Complex Subunit 3, Anaphase-promoting complex subunit 3, APC 3, APC3, APC-3, Cdc 27, Cdc27, Cdc-27, CDC27 homolog, CDC27_HUMAN, CDC27Hs, Cell division cycle 27, Cell division cycle protein 27 homolog, H NUC, H-NUC, HNUC, NUC2 homolog.

Background: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Gene ID: 996