BRCA2 antibody

Catalog No. ABIN8093020

This Mouse Monoclonal antibody specifically detects BRCA2 in WB. It exhibits reactivity toward Human.

Quick Overview for BRCA2 antibody (ABIN8093020)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1847CT631-23-58

Product Details anti-BRCA2 Antibody

Purpose: BRCA2

Purification: Protein G purified

Immunogen: Recombinant human BRCA2 was used as the immunogen for the BRCA2 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the BRCA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the BRCA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2

Background: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, and is required to prevent R-loop- associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:< a href=http://www.uniprot.org/citations/24896180 target=_blank>24896180). [UniProt]

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location