HNF1B antibody (AA 1-200)

Catalog No. ABIN8093246

The Mouse Monoclonal anti-HNF1B antibody is suitable to detect HNF1B in samples from Human. It has been validated for IHC.

Quick Overview for HNF1B antibody (AA 1-200) (ABIN8093246)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HNF1B-9519

Product Details anti-HNF1B Antibody

Binding Specificity: AA 1-200

Purpose: HNF1B Antibody / Transcription factor HNF1 beta

Immunogen: A recombinant fragment (around amino acids 1-200) of human HNF1B protein (exact sequence is proprietary) was used as the immunogen for the TCF2/HNF1B antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the TCF2/HNF1B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: TCF2/HNF1B antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: HNF1B

Background: TCF2 antibody, also known as Hepatocyte nuclear factor 1 beta antibody, recognizes a homeodomain-containing transcription factor encoded by the HNF1B gene. This protein is commonly referred to as HNF1 beta and was historically described as TCF2 in early genetic studies. HNF1B is a nuclear transcription factor that regulates gene expression during embryonic development and adult tissue homeostasis, particularly in liver, kidney, pancreas, and genitourinary epithelium.

HNF1B belongs to the homeodomain-containing transcription factor family and functions as either a homodimer or heterodimer with HNF1A. It binds specific DNA promoter sequences and regulates genes involved in epithelial differentiation, glucose metabolism, and organ morphogenesis. The protein contains a dimerization domain, a POU-specific DNA binding domain, and a transactivation domain that mediates transcriptional regulation of downstream targets.

During development, HNF1B plays a critical role in renal tubulogenesis, pancreatic organogenesis, and hepatobiliary differentiation. It is highly expressed in renal tubular epithelial cells, bile ducts, pancreatic ductal cells, and certain epithelial-derived tissues. Disruption of HNF1B expression alters nephron segmentation and epithelial polarity, highlighting its role in tissue architecture and functional maturation.

Clinically, mutations in HNF1B are associated with Renal cysts and diabetes syndrome, also known as MODY5, as well as congenital abnormalities of the kidney and urinary tract. Loss or altered expression has also been described in subsets of renal cell carcinoma and gynecologic malignancies. Because of its consistent nuclear localization and epithelial lineage specificity, TCF2 antibody is widely used for evaluating HNF1B expression in developmental biology and oncology research.

In normal tissues, HNF1B localizes predominantly to the nucleus, consistent with its transcription factor function. Altered nuclear expression patterns may reflect dysregulation of epithelial differentiation programs. A TCF2 antibody can be used in research applications to detect HNF1B protein expression in relevant experimental systems.

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation