Indian Hedgehog antibody (AA 227-360)

Catalog No. ABIN8093262

The Rabbit Polyclonal anti-Indian Hedgehog antibody (ABIN8093262) specifically detects Indian Hedgehog in WB, ELISA, IHC, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Indian Hedgehog antibody (AA 227-360) (ABIN8093262)

Target: Indian Hedgehog (IHH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Indian Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Indian Hedgehog Antibody

Binding Specificity: AA 227-360

Purpose: IHH Antibody / Indian hedgehog protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Indian hedgehog protein recombinant protein (amino acids D227-A360) was used as the immunogen for the IHH antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IHH antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IHH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Indian Hedgehog

Target: Indian Hedgehog (IHH)

Alternative Name: IHH

Background: IHH antibody targets Indian hedgehog protein, encoded by the IHH gene. Indian hedgehog protein is a secreted signaling molecule belonging to the hedgehog family and plays a central role in embryonic development and tissue patterning. IHH is synthesized as a precursor protein that undergoes autocatalytic cleavage to generate an active N-terminal signaling fragment responsible for pathway activation.

Functionally, Indian hedgehog protein regulates cell proliferation and differentiation through activation of the hedgehog signaling pathway. By interacting with Patched receptors and modulating downstream Gli transcription factors, IHH coordinates communication between neighboring cell populations. An IHH antibody supports studies focused on morphogen signaling and developmental regulation.

IHH expression is especially prominent in skeletal tissues, where it is produced by prehypertrophic chondrocytes during bone development. In this context, Indian hedgehog protein governs the balance between cartilage growth and maturation by coordinating feedback signaling with surrounding perichondrial cells. This spatially restricted expression highlights its importance in endochondral ossification.

From a disease-relevance perspective, dysregulation of Indian hedgehog protein signaling has been linked to skeletal abnormalities and growth plate disorders. Aberrant IHH pathway activity has also been examined in cancer and fibrotic disease, where reactivation of developmental signaling contributes to pathological tissue remodeling.

At the molecular level, Indian hedgehog protein undergoes lipid modification that influences secretion, signaling range, and gradient formation. These processing events can affect its apparent behavior in biochemical assays without altering the primary sequence. IHH antibody reagents support research applications focused on developmental biology and hedgehog pathway signaling, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q14623

Pathways: Hedgehog Signaling