Recombinant MCM5 antibody (AA 500-734)

Catalog No. ABIN8093322

The Mouse Monoclonal anti-MCM5 antibody (Clone rMCM5-13646) (ABIN8093322) specifically detects MCM5 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant MCM5 antibody (AA 500-734) (ABIN8093322)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RMCM5-13646

Product Details anti-MCM5 Antibody

Binding Specificity: AA 500-734

Purpose: MCM5 Antibody / DNA replication licensing factor MCM5

Purification: Protein A affinity

Immunogen: A recombinant fragment (around amino acids 500-734) of human MCM5 protein (exact sequence is proprietary) was used as the immunogen for the recombinant Minichromosome maintenance protein 5/MCM5 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: 1. Optimal dilution of the recombinant Minichromosome maintenance protein 5/MCM5 antibody should be determined by the researcher.

2. This recombinant Minichromosome maintenance protein 5/MCM5 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Minichromosome maintenance protein 5/MCM5 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5

Background: Minichromosome maintenance protein 5 Antibody recognizes MCM5, a nuclear DNA replication factor that functions as a core component of the MCM2-7 helicase complex. Minichromosome maintenance protein 5 antibody, also commonly referred to as MCM5 antibody in replication biology literature, targets a protein essential for origin licensing and replication fork progression. The MCM5 gene encodes one of six conserved subunits that assemble into the heterohexameric replicative helicase required for unwinding double-stranded DNA during S phase.

MCM5 plays a critical role during early G1 phase, when the pre-replication complex is established at replication origins through coordinated recruitment of ORC, CDC6, CDT1, and the MCM2-7 complex. Once activated by cyclin-dependent kinases and DDK, the helicase becomes competent for DNA unwinding, enabling replication fork formation. Because Minichromosome maintenance protein 5 remains associated with chromatin in cells that are replication-licensed, MCM5 antibody identifies cells with proliferative capacity rather than simply cells actively synthesizing DNA.

In normal tissues, MCM5 expression is confined to proliferative compartments such as basal epithelial layers, crypt bases in gastrointestinal mucosa, germinal centers of lymphoid tissue, and stem or progenitor cell populations. Differentiated cells that have exited the cell cycle show minimal nuclear staining. In neoplastic tissues, expanded nuclear expression of MCM5 correlates with increased proliferative fraction and dysregulated cell cycle control. Elevated Minichromosome maintenance protein 5 expression has been documented in colorectal carcinoma, breast carcinoma, lung carcinoma, prostate adenocarcinoma, and various hematologic malignancies, supporting its utility as a proliferation-associated marker in tumor biology research.

Structurally, MCM5 contains conserved ATPase domains characteristic of the AAA+ helicase family and participates directly in ATP-dependent DNA strand separation. Tight regulation of MCM5 activity is essential for preventing re-replication and maintaining genomic integrity. Dysregulation of replication licensing factors such as MCM5 contributes to replication stress and chromosomal instability, hallmarks of oncogenic transformation. Minichromosome maintenance protein 5 Antibody (clone rMCM5/13646) is designed to detect endogenous nuclear MCM5 in research applications, supporting investigation of DNA replication control, cell cycle progression, replication licensing dynamics, and proliferative signaling pathways in both normal and malignant tissues.

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA