MUC16 antibody (AA 1700-2000)

Catalog No. ABIN8093334

The Mouse Monoclonal anti-MUC16 antibody (Clone CA125-8894) (ABIN8093334) specifically detects MUC16 in IHC. The antibody is reactive with Human samples.

Quick Overview for MUC16 antibody (AA 1700-2000) (ABIN8093334)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CA125-8894

Product Details anti-MUC16 Antibody

Binding Specificity: AA 1700-2000

Purpose: MUC16 Antibody / CA125

Immunogen: A recombinant fragment (around amino acids 1700-2000) of human MUC16 protein (exact sequence is proprietary) was used as the immunogen for the CA125 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the CA125 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: CA125 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: MUC16

Background: CA125 Antibody recognizes Mucin 16 (MUC16), a high molecular weight transmembrane glycoprotein widely known as Cancer antigen 125 and Ovarian cancer antigen 125. MUC16 is a membrane-associated mucin characterized by extensive O-linked glycosylation, multiple tandem repeat domains, and a large extracellular region that can be shed into circulation. CA125 Antibody is useful for detecting MUC16 expression in epithelial and tumor tissues in research applications.

MUC16 antibody, also referred to as CA125 antibody and Mucin-16 antibody in the literature, targets a mucin primarily expressed on the apical surface of epithelial cells lining the female reproductive tract, including endometrium and fallopian tube, as well as in ocular and respiratory epithelium. Under physiologic conditions, MUC16 contributes to epithelial barrier protection, lubrication, and defense against mechanical and microbial stress. Proteolytic cleavage of its extracellular domain releases circulating CA125, which forms the basis of the clinically utilized serum biomarker for ovarian carcinoma monitoring.

The MUC16 gene is located on human chromosome 19p13.2 and encodes one of the largest membrane-associated proteins identified. Structurally, the protein contains numerous SEA domains, extensive tandem repeat regions, a transmembrane segment, and a short cytoplasmic tail that participates in intracellular signaling interactions. MUC16 has been shown to interact with mesothelin and other adhesion molecules, facilitating tumor cell attachment, peritoneal dissemination, and immune modulation in ovarian carcinoma.

Aberrant overexpression of MUC16 is strongly associated with epithelial ovarian carcinoma and is also reported in subsets of endometrial, pancreatic, breast, and lung carcinomas. In histologic sections, MUC16 typically demonstrates apical membranous and cytoplasmic staining in positive epithelial tumor cells. A CA125 antibody is commonly applied in research settings to study epithelial differentiation, tumor origin, and mucin biology. Clone CA125/8894 is a mouse monoclonal antibody developed to target MUC16 in experimental systems.

UniProt: Q8WXI7