Recombinant MUC16 antibody

Catalog No. ABIN8093341

This Rabbit Monoclonal antibody specifically detects MUC16 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant MUC16 antibody (ABIN8093341)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: CA125-13895R

Product Details anti-MUC16 Antibody

Purpose: MUC16 Antibody / Ovarian cancer antigen 125 (azide and preservative free)

Purification: Protein A affinity

Immunogen: Recombinant human MUC16 protein was used as the immunogen for the Ovarian cancer antigen 125 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the Ovarian cancer antigen 125 antibody should be determined by the researcher.

2. This Ovarian cancer antigen 125 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Ovarian cancer antigen 125 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: MUC16

Background: Ovarian cancer antigen 125 Antibody recognizes Mucin 16 (MUC16), a very high molecular weight transmembrane glycoprotein widely known as CA125 and cancer antigen 125. MUC16 is a membrane-associated mucin characterized by extensive O-linked glycosylation, multiple tandem repeat domains, and a large extracellular region that can be proteolytically shed into circulation. Ovarian cancer antigen 125 Antibody is designed for detection of MUC16 expression in epithelial and tumor tissues in research applications.

MUC16 antibody, also referred to as CA125 antibody and Mucin-16 antibody in the literature, targets a mucin primarily expressed on the apical surface of epithelial cells lining the female reproductive tract, including endometrium and fallopian tube, as well as in ocular and respiratory epithelium. Under physiologic conditions, MUC16 contributes to epithelial barrier protection, lubrication, and defense against environmental stress. Proteolytic release of its extracellular domain produces circulating CA125, which is widely used as a serum biomarker for monitoring ovarian carcinoma.

The MUC16 gene is located on human chromosome 19p13.2 and encodes one of the largest membrane-associated proteins identified. Structurally, MUC16 contains numerous SEA domains, extensive tandem repeat regions, a transmembrane segment, and a short cytoplasmic tail involved in intracellular signaling interactions. MUC16 has been shown to bind mesothelin and facilitate tumor cell adhesion and peritoneal dissemination in ovarian carcinoma models.

Aberrant overexpression of MUC16 is most strongly associated with epithelial ovarian carcinoma and is also reported in subsets of endometrial, pancreatic, breast, lung, and gastrointestinal carcinomas. In histologic sections, MUC16 typically demonstrates apical membranous and cytoplasmic staining in positive epithelial tumor cells. An ovarian cancer antigen 125 antibody is commonly applied in research settings to evaluate epithelial differentiation, tumor origin, and mucin biology. Clone CA125/13895R is a recombinant rabbit monoclonal antibody developed to target MUC16 in experimental systems.

UniProt: Q8WXI7