PEX5 antibody (Middle Region)

Catalog No. ABIN8093406

The Rabbit Polyclonal anti-PEX5 antibody (ABIN8093406) specifically detects PEX5 in WB, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PEX5 antibody (Middle Region) (ABIN8093406)

Target: PEX5 (Peroxisomal Biogenesis Factor 5 (PEX5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PEX5 Antibody

Binding Specificity: Middle Region

Purpose: PEX5 Antibody / Peroxisomal biogenesis factor 5

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Peroxisomal biogenesis factor 5 was used as the immunogen for the PEX5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PEX5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PEX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PEX5

Target: PEX5 (Peroxisomal Biogenesis Factor 5 (PEX5))

Alternative Name: PEX5

Background: PEX5 antibody targets Peroxisomal biogenesis factor 5, encoded by the PEX5 gene. Peroxisomal biogenesis factor 5 is a cytoplasmic protein that functions as the primary receptor for peroxisomal targeting signal 1 (PTS1) containing proteins. By recognizing the C-terminal PTS1 motif on newly synthesized matrix proteins, PEX5 mediates their delivery from the cytosol to the peroxisomal membrane for import into the peroxisome matrix.

Functionally, Peroxisomal biogenesis factor 5 operates as a shuttling receptor that binds cargo proteins in the cytoplasm and docks at the peroxisomal membrane through interactions with other peroxins. Following cargo release into the peroxisome, PEX5 is recycled back to the cytosol, allowing repeated rounds of protein import. A PEX5 antibody supports studies focused on peroxisomal protein trafficking, organelle biogenesis, and intracellular transport mechanisms.

PEX5 is predominantly localized to the cytoplasm but transiently associates with the peroxisomal membrane during active import cycles. Its dynamic localization reflects its role as a transport receptor rather than a static structural component of the organelle. Through regulation of matrix protein import, Peroxisomal biogenesis factor 5 supports essential peroxisomal functions, including fatty acid beta-oxidation, lipid metabolism, and reactive oxygen species detoxification.

From a disease-related perspective, disruption of PEX5 function has been linked to peroxisome biogenesis disorders, in which impaired import of matrix enzymes leads to severe metabolic dysfunction. Altered expression or activity of PEX5 can compromise peroxisomal integrity and downstream metabolic pathways, making it an important target in studies of inherited metabolic disease and organelle dysfunction.

At the molecular level, Peroxisomal biogenesis factor 5 contains conserved domains required for PTS1 recognition and interaction with peroxisomal membrane components. Its apparent behavior in experimental systems may vary with cellular metabolic state and import demand without changes to its primary structure. PEX5 antibody reagents enable investigation of peroxisomal protein import and organelle biogenesis, with NSJ Bioreagents providing reagents intended for research use.

UniProt: P50542

Pathways: Monocarboxylic Acid Catabolic Process