SYVN1 antibody (AA 68-604)

Catalog No. ABIN8093504

The Rabbit Polyclonal anti-SYVN1 antibody is suitable to detect SYVN1 in samples from Human, Mouse and Rat. It has been validated for WB, FACS, IF, IP and ICC.

Quick Overview for SYVN1 antibody (AA 68-604) (ABIN8093504)

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYVN1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SYVN1 Antibody

Binding Specificity: AA 68-604

Purpose: SYVN1 Antibody / Synoviolin / HRD1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human HRD1/SYVN1 recombinant protein (amino acids Q68-R604) was used as the immunogen for the Synoviolin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Synoviolin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Synoviolin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SYVN1

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Alternative Name: SYVN1

Background: Synoviolin antibody targets Synoviolin (SYVN1), also known as HRD1, an endoplasmic reticulum membrane-anchored E3 ubiquitin ligase that functions as a central component of the ER-associated degradation pathway. Synoviolin mediates the ubiquitination of misfolded or excess proteins within the endoplasmic reticulum, marking them for retrotranslocation and proteasomal degradation. The protein localizes predominantly to the ER membrane, where its cytosolic RING finger domain catalyzes ubiquitin transfer in coordination with E2 enzymes. Through this activity, Synoviolin plays a key role in maintaining ER protein quality control and cellular proteostasis.

Functionally, Synoviolin regulates ER homeostasis by limiting the accumulation of aberrant proteins that would otherwise trigger prolonged unfolded protein response signaling. By promoting efficient clearance of defective substrates, Synoviolin helps balance protein synthesis with folding capacity and prevents chronic ER stress. Synoviolin expression is broadly observed across tissues, reflecting the universal requirement for ER quality control in secretory and metabolically active cells. A Synoviolin antibody supports studies examining ubiquitin-dependent protein degradation and ER stress regulation.

Synoviolin has been implicated in controlling the stability of specific regulatory proteins involved in cell survival, inflammation, and metabolic signaling. Altered Synoviolin activity can influence cellular responses to stress by modulating degradation rates of ER-resident and ER-associated substrates. In experimental systems, changes in Synoviolin levels affect sensitivity to ER stressors and perturbations in protein folding demand, highlighting its role as a gatekeeper of ER proteostasis.

From a biological and disease-relevance perspective, Synoviolin has been studied in the context of inflammatory disease, fibrosis, and cancer biology, where dysregulated protein quality control contributes to pathogenesis. HRD1-mediated ERAD activity influences inflammatory signaling pathways and cell fate decisions under stress conditions. Understanding Synoviolin expression and regulation provides insight into how cells adapt to chronic ER stress and maintain homeostasis under pathological challenges.

At the molecular level, Synoviolin is encoded by the SYVN1 gene and produces a protein of approximately 617 amino acids. The protein contains multiple transmembrane domains anchoring it to the ER membrane and a cytosolic RING finger motif required for E3 ubiquitin ligase activity. Regulation of Synoviolin occurs through transcriptional control, protein interactions, and feedback from ER stress signaling pathways. A Synoviolin antibody supports research applications focused on ER-associated degradation, ubiquitin signaling, and cellular stress responses, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q86TM6

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling