Recombinant Uroplakin 2 antibody (AA 36-51)

Catalog No. ABIN8093553

The Rabbit Monoclonal anti-Uroplakin 2 antibody (Clone UPK2-13669R) (ABIN8093553) specifically detects Uroplakin 2 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Uroplakin 2 antibody (AA 36-51) (ABIN8093553)

Target: Uroplakin 2 (UPK2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Uroplakin 2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: UPK2-13669R

Product Details anti-Uroplakin 2 Antibody

Binding Specificity: AA 36-51

Purpose: UPK2 Antibody / Uroplakin 2

Purification: Protein A affinity

Immunogen: A recombinant fragment (around amino acids 36-51) of human Uroplakin 2 (UPK2) protein (exact sequence is proprietary) was used as the immunogen for the UPK2/Uroplakin 2 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the UPK2/Uroplakin 2 antibody should be determined by the researcher.

2. This UPK2/Uroplakin 2 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: UPK2/Uroplakin 2 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Uroplakin 2

Target: Uroplakin 2 (UPK2)

Alternative Name: UPK2

Background: UPK2 antibody recognizes Uroplakin 2, a transmembrane protein encoded by the human UPK2 gene and a critical structural component of the urothelial plaque. Uroplakin 2 is specifically expressed in the umbrella cells of the urothelium lining the urinary bladder, ureters, and renal pelvis. As a differentiation marker of urothelial cells, UPK2 antibody is widely used in research focused on urothelial biology and bladder carcinoma characterization.

Uroplakin 2 belongs to the uroplakin family, which includes UPK1A, UPK1B, UPK2, and UPK3A. These proteins assemble into heterodimeric complexes that form highly organized crystalline plaques on the apical surface of urothelial umbrella cells. Uroplakin 2 pairs with Uroplakin 1A to form a heterodimer that is subsequently incorporated into larger hexagonal arrays. These plaques create a permeability barrier that protects underlying tissues from urine toxicity and mechanical stress. Detection of Uroplakin 2 therefore reflects terminal urothelial differentiation and apical membrane specialization.

UPK2 is localized to the apical plasma membrane of superficial umbrella cells, where it contributes to plaque rigidity and barrier integrity. Expression is largely restricted to urothelial tissue, making Uroplakin 2 a highly specific marker for cells of urothelial origin. In pathologic settings, UPK2 expression is maintained in many urothelial carcinomas, including invasive bladder carcinoma, and can assist in distinguishing urothelial tumors from other carcinomas in metastatic contexts.

The UPK2 gene is located on chromosome 11q23 and encodes a glycosylated membrane protein that undergoes processing and assembly within the endoplasmic reticulum prior to trafficking to the apical membrane. UPK2 antibody clone UPK2/13669R is a recombinant rabbit monoclonal antibody developed to detect Uroplakin 2 in research applications, supporting studies of urothelial differentiation and tumor origin assessment.

UniProt: O00526