Recombinant Uroplakin 2 antibody (AA 36-51)

Catalog No. ABIN8093555

The Rabbit Monoclonal anti-Uroplakin 2 antibody is suitable to detect Uroplakin 2 in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant Uroplakin 2 antibody (AA 36-51) (ABIN8093555)

Target: Uroplakin 2 (UPK2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Uroplakin 2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: UPK2-13348R

Product Details anti-Uroplakin 2 Antibody

Binding Specificity: AA 36-51

Purpose: UPK2 Antibody / Uroplakin 2

Purification: Protein A affinity

Immunogen: A recombinant fragment (around amino acids 36-51) of human Uroplakin 2 (UPK2) protein (exact sequence is proprietary) was used as the immunogen for the Uroplakin 2/UPK2 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: 1. Optimal dilution of the Uroplakin 2/UPK2 antibody should be determined by the researcher.

2. This Uroplakin 2/UPK2 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Uroplakin 2/UPK2 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for Uroplakin 2

Target: Uroplakin 2 (UPK2)

Alternative Name: UPK2

Background: Uroplakin 2 antibody recognizes Uroplakin 2, a urothelial differentiation protein encoded by the human UPK2 gene and a key structural component of the urothelial plaque. Uroplakin 2, also referred to as UPK2, is selectively expressed in the superficial umbrella cells of the urothelium lining the urinary bladder, ureters, and renal pelvis. Because of its restricted tissue distribution, Uroplakin 2 antibody is widely used in research investigating urothelial biology and bladder carcinoma.

Uroplakin 2 is a member of the uroplakin family, which includes UPK1A, UPK1B, UPK2, and UPK3A. These proteins assemble into heterodimeric complexes that form highly ordered crystalline plaques on the apical surface of umbrella cells. Uroplakin 2 pairs specifically with Uroplakin 1A, forming a heterodimer that is incorporated into larger hexagonal arrays within the asymmetric unit membrane. These plaques provide structural rigidity and create a permeability barrier that protects underlying tissues from urine toxicity and mechanical stress. Detection of Uroplakin 2 therefore reflects terminal urothelial differentiation and specialized apical membrane architecture.

UPK2 is localized predominantly to the apical plasma membrane of differentiated umbrella cells, where it contributes to barrier integrity and bladder surface stability. Expression is largely confined to urothelial tissue, making Uroplakin 2 a highly specific marker of urothelial origin. In pathologic settings, UPK2 expression is retained in many urothelial carcinomas, including invasive bladder carcinoma and metastatic urothelial tumors, supporting its utility in studies of tumor lineage and differentiation status.

The UPK2 gene is located on chromosome 11q23 and encodes a glycosylated membrane protein that undergoes post-translational processing and assembly within the endoplasmic reticulum before trafficking to the apical surface. Uroplakin 2 antibody clone UPK2/13348R is a recombinant rabbit monoclonal antibody developed to detect UPK2 in research applications, supporting studies of urothelial differentiation and bladder tumor characterization.

UniProt: O00526