Actin antibody (AA 300-377)

Catalog No. ABIN8119496

This Mouse Monoclonal antibody specifically detects Actin in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Rat, Mouse, Chicken, Cow and Pig.

Quick Overview for Actin antibody (AA 300-377) (ABIN8119496)

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: AB1489

Product Details anti-Actin Antibody

Binding Specificity: AA 300-377

Purpose: Actin pan mouse mAb

Specificity: This antibody detects endogenous levels of all actins protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 300 - 377 of the human Actin pan protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Actin

Target: Actin

Alternative Name: Actin pan

Background:

Synonyms: Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC, Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, Actin, aortic smooth muscle, Alpha-actin-2, ACTA2, ACTSA, ACTVS, Actin, cytoplasmic 1, Beta-actin, ACTB, Actin, cytoplasmic 2, Gamma-actin, ACTG1, ACTG, Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin, ACTA3, ACTL3, ACTSG, ACTG2

Background: The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008],

Gene Name: ACTC1 ACTA1 ACTA2 ACTB ACTG1 ACTG2

Protein Name: Actin pan

Gene ID: 70, 58, 59

UniProt: P62736