Actin antibody

Catalog No. ABIN8119500

The Rabbit Monoclonal anti-Actin antibody is suitable to detect Actin in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF, ELISA and IP.

Quick Overview for Actin antibody (ABIN8119500)

Target: Actin

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Clone: AB1724

Product Details anti-Actin Antibody

Purpose: Actin pan Rabbit mAb

Specificity: This antibody detects endogenous levels of Actin pan protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for Actin

Target: Actin

Alternative Name: Actin pan

Background:

Synonyms: Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC, Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, Actin, aortic smooth muscle, Alpha-actin-2, ACTA2, ACTSA, ACTVS, Actin, cytoplasmic 1, Beta-actin, ACTB, Actin, cytoplasmic 2, Gamma-actin, ACTG1, ACTG, Actin, gamma-enteric smooth muscle, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin, ACTA3, ACTL3, ACTSG, ACTG2

Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],

Gene Name: Actin-pan

Protein Name: Actin pan

Gene ID: 59, 58, 60

UniProt: P62736