PRAS40 antibody (pThr246)

Catalog No. ABIN8119640

This Rabbit Monoclonal antibody specifically detects PRAS40 in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRAS40 antibody (pThr246) (ABIN8119640)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2207

Product Details anti-PRAS40 Antibody

Binding Specificity: pThr246

Purpose: PRAS40 (Phospho Thr246) Rabbit mAb

Specificity: The antibody detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246. The antibody does not cross-react with PRAS40 phosphorylated at other sites.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: PRAS40

Background:

Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

Background: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. , 2003 [PubMed 12524439]) . [supplied by OMIM, Mar 2008],

Gene Name: AKT1S1

Protein Name: PRAS40 (Phospho Thr246)

Gene ID: 84335

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect