ALOX5 antibody (pSer523)

Catalog No. ABIN8119785

The Rabbit Polyclonal anti-ALOX5 antibody is suitable to detect ALOX5 in samples from Human and Rat. It has been validated for WB and ELISA.

Quick Overview for ALOX5 antibody (pSer523) (ABIN8119785)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ALOX5 Antibody

Binding Specificity: pSer523

Purpose: 5-LO (phospho Ser523) Antibody

Specificity: Phospho-5-LO (S523) Polyclonal Antibody detects endogenous levels of 5-LO protein only when phosphorylated at S523.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human 5-LO (phospho Ser523)

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: 5-LO

Background:

Synonyms: ALOX5, LOG5, Arachidonate 5-lipoxygenase, 5-LO, 5-lipoxygenase

Background: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

Gene Name: ALOX5

Protein Name: Arachidonate 5-lipoxygenase

Gene ID: 240

UniProt: P09917