ARNTL antibody

Catalog No. ABIN8119814

This Rabbit Monoclonal antibody specifically detects ARNTL in WB, ELISA, IHC, IF and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ARNTL antibody (ABIN8119814)

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ARNTL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2158

Product Details anti-ARNTL Antibody

Purpose: BMAL1 Rabbit mAb

Specificity: This antibody detects endogenous levels of BMAL1 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for ARNTL

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Alternative Name: BMAL1

Background:

Synonyms: ARNTL, BHLHE5, BMAL1, MOP3, PASD3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1Class E basic helix-loop-helix protein 5, bHLHe5, Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3

Background: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],

Gene Name: ARNTL

Protein Name: BMAL1

Gene ID: 406

UniProt: O00327

Pathways: Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effect