BID antibody (Cleaved-Ser100)

Catalog No. ABIN8119995

The Rabbit Polyclonal anti-BID antibody is suitable to detect BID in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for BID antibody (Cleaved-Ser100) (ABIN8119995)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BID Antibody

Binding Specificity: Cleaved-Ser100

Purpose: BID (p11, Cleaved-Ser100) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse BID (p11, Cleaved-Ser100, protein was cleaved amino acid sequence between 99-100)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human BID (p11, Cleaved-Ser100)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background:

Synonyms: BH3-interacting domain death agonist (p22 BID, BID) [Cleaved into: BH3-interacting domain death agonist p15 (p15 BID), BH3-interacting domain death agonist p13 (p13 BID), BH3-interacting domain death agonist p11 (p11 BID)]

Background: domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.,subcellular location:A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.,subcellular location:Associated with the mitochondrial membrane.,subcellular location:Translocates to mitochondria as an integral membrane protein.,subcellular location:When uncleaved, it is predominantly cytoplasmic.,subunit:Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2.,tissue specificity:Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).,

Gene Name: BID

Protein Name: BID (p11, Cleaved-Ser100)

Gene ID: 637

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity