BRCA2 antibody (pSer3291)

Catalog No. ABIN8120033

This Rabbit Polyclonal antibody specifically detects BRCA2 in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for BRCA2 antibody (pSer3291) (ABIN8120033)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BRCA2 Antibody

Binding Specificity: pSer3291

Purpose: BRCA2 (phospho Ser3291) Antibody

Specificity: Phospho-BRCA2 (S3291) Polyclonal Antibody detects endogenous levels of BRCA2 protein only when phosphorylated at S3291.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human BRCA2 (phospho Ser3291)

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2

Background:

Synonyms: BRCA2, FACD, FANCD1, Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein

Background: Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008],

Gene Name: BRCA2

Protein Name: Breast cancer type 2 susceptibility protein

Gene ID: 675

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location